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زیست شناسی::
اندونوکلئاز محدود کننده
A leucinerequiring (leu2) yeast strain is transformed with restriction endonuclease digested vector DNA because chromosomal DNA is more likely to recombine with linearized DNA than with circular DNA.
The restriction endonuclease (RE) sites flank the segments from the nonessential gene.
Moreover, the induction of the cloned gene can be timed to maximize re combinant protein production during largescale fermentations.
(A) A single recombination (dashed line) between the HIS4 gene of an intact circular plasmid and a chromosome his4 mutant gene re sults in the integration of the entire vector, including the gene of interest (GOI) with the AOX1 promoter in the 5?
AOX1 DNA segments of a restriction endonuclease (RE) linearized DNA fragment from the vector and the corresponding chromosome regions results in the integration of the gene of interest (GOI) with the AOX1 promoter in the 5?
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